Enzymes and Inhibitors
Abcam Bluo-Gal, Chromogenic beta-galactosidase substrate, 250MG
MW 374.2 g/mol, Purity >98%. Chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Designed as an alternative to X-Gal (ab144388) in blue-white selection of recombinant bacterial colonies with the lac+ phenotype, producing a darker blue color.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Nitrocefin, Chromogenic cephalosporin beta-lactamase substrate, 5MG
MW 516.5 Da, Purity >95%. Chromogenic cephalosporin β-lactamase substrate. Indicates β-lactamase activity via a red color intensely proportional to the original concentration of Nitrocefin. Undergoes rapid distinctive color change from yellow (max at pH 7.0 = 390 nm) to red (max at pH 7.0 = 486 nm) as the amide bond in the beta-lactam ring is hydrolyzed.
The product is subject to the following: Abcam Restricted Use Statement
Abcam O x iRed™ probe, pero x idase fluorogenic substrate, 5MG
MW 257.24 Da, Purity >99%. Highly sensitive, stable peroxidase fluorogenic substrate used as a hydrogen peroxide probe.
The product is subject to the following: Abcam Restricted Use Statement
INTACT GENOMICS INC Endonuclease IV (Nfo) 2000 Units (10 units/µl)
Endonuclease IV (Nfo) from Escherichia coli is a 32-kD metalloprotein that aids in the repair of damaged DNA. The enzyme functions both as an apurinic/apyrimidinic nuclease (1) and as a 3′-terminal di-esterase (1-4). Its 3′-terminal di-esterase activity is important in the repair of DNA strand breaks generated by oxidation and ionic radiation (2, 3). In such events, the strand breaks terminate with either a 3′ phosphate or a deoxyribose fragment, preventing repair by DNA polymerase I or DNA ligase. Endonuclease IV removes the blocking groups, leaving a free 3′-hydroxyl terminus. This enzyme does not have detectable associated exonuclease or DNA N-glycosylase activity (1).Applications• Single cell gel electrophoresis (Comet assay) (5, 6)• Alkaline elution (7)• Alkaline unwinding (8)Product SourceE. coli BL21 (DE3) strain expressing E. coli Endonuclease IV gene.Product Includes• Endonuclease IV (Nfo)• 10x Endonuclease IV reaction buffer
ABclonal Technology Exonuclease III (E. coli)
Exonuclease III (E.coli) catalyzes the stepwise removal of mononucleotides from 3?-hydroxyl termini of duplex DNA. The preferred substrates are dsDNA with blunt or recessed 3?-, termini although the enzyme also acts at nicks in dsDNA to produce single-strand gaps. dsDNA with 3?-overhang are resistant to cleavage; when overhangs length is ≥ 4 bases，dsDNA are able to fully prevent ExoIII cleavage process. Exonuclease III has also been reported to have RNase H, 3?-phosphatase and AP-endonuclease activities.
Medchemexpress LLC Multi-kinase inhibitor 1 | 778274-97-8 | 99.1% | 418.37 g·mol⁻¹ | C20H17F3N4O3 | 1 ML
Multi-kinase inhibitor 1 is a small-molecule multi-kinase inhibitor provided as a ready-to-use 10 mM solution in DMSO for laboratory research. It has molecular formula C20H17F3N4O3, a molecular weight of 418.37 g·mol⁻¹, and CAS number 778274-97-8. Intended for research use only.
- Ready-to-use 10 mM solution in DMSO.
- High purity (99.05%).
- Molecular weight 418.37 g·mol⁻¹.
- CAS 778274-97-8.
- Supplied as 1 mL vial for single-use experiments.
Sigma Aldrich Fine Chemicals Biosciences alpha-Amylase Bacillu250ML
Amylase belongs to glycosyl hydrolase family 13. It comprises of A B and C domains. Domain A has two aspartic acid and a glutamic acid as catalytic residues. Domain B possesses Ca2 and Na binding sites.
Sigma Aldrich Fine Chemicals Biosciences Endoglycosidase F2 from Elizabethkingia miricola recombinant, expressed in E. coli, 20 U/mg | 37278-88-9 | MFCD00151069 | 0.2UN
Endoglycosidase F2 from Elizabethkingia miricola recombinant, expressed in E. coli, 20 U/mg | 37278-88-9 | MFCD00151069 | 0.2UN
Abcam D-(+)-Cellobiose, beta-glucosidase substrate, 5G
MW 342.3 g/mol, Purity >99%. Disaccharide consisting of two β-glucose molecules linked by a β-1,4 glycosidic bond. Used as a substrate of β-glucosidase.
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC Her2/cd340 protein, rhesus macaque (HEK293, His) | >95.0% | 10 UG
Recombinant HER2/CD340 protein derived from rhesus macaque, expressed in HEK293 cells and supplied with a C-terminal His tag. Purified (>95% purity) and formulated for use in biochemical assays, antibody development, binding studies, and assay controls.
- Recombinant extracellular domain of HER2 protein.
- Derived from rhesus macaque.
- Expressed in HEK293 cells.
- C-terminal His tag for purification and detection.
- Purity greater than 95% by reducing SDS-PAGE.
- Available in microgram-scale quantities, including 10 μg.
- Store at -20°C; after reconstitution stable at 4°C for 1 week.
- Suitable for biochemical assays, antibody development, and binding studies.
Enzo Life Sciences Sinefungin (5mg). CAS: 58944-73-3
Strong competitive inhibitor of methyltransferases which uses S-adenosyl-1-methionine as the methyl group donor to yield methylated products such as 5-methylcytosine or N6-methyl adenosine on DNA and RNA. In addition, sinefungin is involved in a number of physiological processes. Alternative name: 6,9-Diamino-1-(6-amino-9H-purin-9-yl)-1,5,6,7,8,9-hexadeoxy-D-glycero-alpha-L-talo-decafuranuronic acid, A-9145, Adenosylornithine. Purity: ≥95% (HPLC). Appearance: White to yellow powder. Solubility: Soluble in water. Long Term Storage: -20°C.
Enzo Life Sciences BAADT (5 mg)
Granzyme B substrate. Chromogenic substrate for granzyme B. λmax is at 405nm. Alternative Name: Granzyme B substrate (chromogenic), Boc-AAD-SBzl. Formula: C22H31N3O7S. MW: 481.6. Purity: ≥95% (TLC). Sequence Boc-Ala-Ala-Asp-SBzl. Appearance: White to off-white crystalline powder. Solubility: Soluble in DMSO. Long Term Storage: -20°C. Handling: Very hygroscopic.
Sigma Aldrich Fine Chemicals Biosciences Catalase from bovine liver aqueous suspension, 10,000-40,000 units/mg protein | 9001-05-2 | MFCD00081483 | 1g
Catalase from bovine liver aqueous suspension, 10,000-40,000 units/mg protein | 9001-05-2 | MFCD00081483 | 1g
Sigma Aldrich Fine Chemicals Biosciences Peroxidase from horseradish Type VI, essentially salt-free, lyophilized powder, >=250 units/mg solid (using pyrogallol) | 9003-99-0 | MFCD00071339 | 5KU
Peroxidase from horseradish Type VI, essentially salt-free, lyophilized powder, >=250 units/mg solid (using pyrogallol) | 9003-99-0 | MFCD00071339 | 5KU